Data/hora: 07/06/2024 05:19:08

Provedor de dados: | 56
País: | Brazil
Título: | Distribution of versican and hyaluronan in the mouse uterus during decidualization
Autores: | San Martin,S.; Soto-Suazo,M.; Zorn,T.M.T.
Data: | 2003-08-01
Ano: | 2003
Palavras-chave: | Decidualization; Endometrium; Proteoglycans; Versican; Hyaluronan; Mouse.
Resumo: | Preparation for embryo implantation requires extensive adaptation of the uterine microenvironment. This process consists of cell proliferation and cell differentiation resulting in the transformation of endometrial fibroblasts into a new type of cell called decidual cell. In the present study, we followed the space-time distribution of versican and hyaluronan (HA) in different tissues of the uterus before and after embryo implantation. Fragments of mouse uteri obtained on the fourth, fifth, sixth and seventh days of pregnancy were fixed in Methacarn, embedded in Paraplast and cut into 5-µm thick sections. HA was detected using a biotinylated fragment of the proteoglycan aggrecan, which binds to this glycosaminoglycan with high affinity and specificity. Versican was detected by a polyclonal antibody. Both reactions were developed by peroxidase methods. Before embryo implantation, both HA and versican were present in the endometrial stroma. However, after embryo implantation, HA disappeared from the decidual region immediately surrounding the implantation chamber, whereas versican accumulated in the same region. The differences observed in the expression of HA and versican suggest that both molecules may participate in the process of endometrial decidualization and/or embryo implantation.
Tipo: | Info:eu-repo/semantics/article
Idioma: | Inglês
Identificador: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2003000800013
Editor: | Associação Brasileira de Divulgação Científica
Relação: | 10.1590/S0100-879X2003000800013
Formato: | text/html
Fonte: | Brazilian Journal of Medical and Biological Research  v.36 n.8 2003
Fonte: | info:eu-repo/semantics/openAccess