Data/hora: 11/06/2024 10:05:55

Provedor de dados: | 56
País: | Brazil
Título: | Two new mixed-ligand coordination polymers based on multi-N chelating ligand inhibit YAP expression and induce caspase-mediated spinal tumor cell apoptosis
Autores: | Sun,Yi Fu; Shao,Li Wei; Chen,Qi; Gao,Xu; Li,Fang; Wu,Chang Yan.
Data: | 2019-01-01
Ano: | 2019
Palavras-chave: | Coordination polymers; Glioma cell strains; Cytotoxicity; Apoptosis; Immunoblotting.
Resumo: | Two new coordination polymers [Zn (bdc)(bpybzimH2)](DMF)0.5 (1, H2bdc=1,4-dicarboxybenzene, bpybzimH2=6,6′-bis-(1H-benzoimidazol-2-yl)-2,2′-bipyridine, DMF=N,N-dimethylformamide) and [Co (bpybzimH2)(sbc)]H2O (2, H2sbc=4-mercaptobenzoic acid) have been successfully prepared under solvothermal conditions using the multi-N chelating organic ligand bpybzimH2 as the foundational building block. In addition, the Cell Counting Kit-8 assay was conducted to evaluate the anti-proliferation activity of compounds 1 and 2 against human spinal tumor cells OPM-2. The cell viability curves showed that the two compounds have anti-proliferation activity on spinal tumor cells, and the activity of compound 1 is higher than compound 2. The annexin V-FITC/PI assay and western blot were used to detect the apoptotic percentage of OPM-2 cells incubated with compounds 1 and 2. The YAP protein expression and its role in cell apoptosis were further studied with qRT-PCR, immunoblotting, and flow cytometer.
Tipo: | Info:eu-repo/semantics/article
Idioma: | Inglês
Identificador: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2019000500608
Editor: | Associação Brasileira de Divulgação Científica
Relação: | 10.1590/1414-431x20198499
Formato: | text/html
Fonte: | Brazilian Journal of Medical and Biological Research  v.52 n.5 2019
Fonte: | info:eu-repo/semantics/openAccess