Data/hora: 01/06/2024 02:14:16

Provedor de dados: 	56	
País: 	Brazil	
Título: 	Alterations in proteins of bone marrow extracellular matrix in undernourished mice	
Autores: 	Vituri,C.L.; Alvarez-Silva,M.; Trentin,A.G.; Borelli,P.	
Data: 	2000-08-01	
Ano: 	2000	
Palavras-chave: 	Fibronectin; Bone marrow; Extracellular matrix; Malnutrition.	
Resumo: 	The objective of the present study was to determine the effect of protein malnutrition on the glycoprotein content of bone marrow extracellular matrix (ECM). Two-month-old male Swiss mice were submitted to protein malnutrition with a low-protein diet containing 4% casein as compared to 20% casein in the control diet. When the experimental group had attained a 20% loss of their original body weight, we extracted the ECM proteins from bone marrow with PBS buffer, and analyzed ECM samples by SDS-PAGE (7.5%) and ECL Western blotting. Quantitative differences were observed between control and experimental groups. Bone marrow ECM from undernourished mice had greater amounts of extractable fibronectin (1.6-fold increase) and laminin (4.8-fold increase) when compared to the control group. These results suggest an association between fluctuations in the composition of the hematopoietic microenvironment and altered hematopoiesis observed in undernourished mice.	
Tipo: 	Info:eu-repo/semantics/article	
Idioma: 	Inglês	
Identificador: 	http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2000000800004	
Editor: 	Associação Brasileira de Divulgação Científica	
Relação: 	10.1590/S0100-879X2000000800004	
Formato: 	text/html	
Fonte: 	Brazilian Journal of Medical and Biological Research  v.33 n.8 2000	
Fonte: 	info:eu-repo/semantics/openAccess