Data/hora: 07/06/2024 02:55:03

Provedor de dados: 	56	
País: 	Brazil	
Título: 	Expression of CHPF modulates cell proliferation and invasion in lung cancer	
Autores: 	Cao,Chengsong; Liu,Yong; Wang,Qun; Zhao,Jing; Shi,Ming; Zheng,Junnian.	
Data: 	2020-01-01	
Ano: 	2020	
Palavras-chave: 	CHPF; Lung cancer; Proliferation; Invasion; TCGA; Affymetrix Gene Chip.	
Resumo: 	Lung cancer is the most common malignancy worldwide and is characterized by rapid progression, aggressive behavior, frequent recurrence, and poor prognosis. The TCGA database indicates that chondroitin polymerizing factor (CHPF) is overexpressed in human lung cancer tissues compared with normal tissues and this overexpression corresponds to shorter overall survival in lung cancer patients. In this study, to investigate the function of CHPF in lung cancer, lentiviral vectors expressing CHPF shRNA were stably transduced into A549 and H1299 cells. Compared to shCtrl cells, CHPF knockdown cells had significantly reduced proliferation. Furthermore, the silencing of CHPF in A549 and H1299 cells resulted in apoptotic induction, which led to decreased colony formation. Wound healing and transwell invasion assays revealed that CHPF could positively regulate the migration of lung cancer cells. The tumorigenic role of CHPF was also validated in nude mouse xenograft models. Affymetrix gene chip analysis indicated that CHPF regulated the proliferation and invasion of lung cancer cells through CDH1, RRM2, MKI67, and TNFRSF10B. We thus highlight CHPF as a novel target for lung cancer treatment.	
Tipo: 	Info:eu-repo/semantics/article	
Idioma: 	Inglês	
Identificador: 	http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2020000500610	
Editor: 	Associação Brasileira de Divulgação Científica	
Relação: 	10.1590/1414-431x20209021	
Formato: 	text/html	
Fonte: 	Brazilian Journal of Medical and Biological Research  v.53 n.5 2020	
Fonte: 	info:eu-repo/semantics/openAccess