Data/hora: 06/06/2024 04:28:46

Provedor de dados: | 56
País: | Brazil
Título: | The effects of 6-gingerol on proliferation, differentiation, and maturation of osteoblast-like MG-63 cells
Autores: | Fan,J.Z.; Yang,X.; Bi,Z.G.
Data: | 2015-07-01
Ano: | 2015
Palavras-chave: | 6-Gingerol; Interleukin-6; Nuclear factor-κB; Osteogenesis; Osteoblast.
Resumo: | We investigated whether 6-gingerol affects the maturation and proliferation of osteoblast-like MG63 cells in vitro. Osteoblast-like MG63 cells were treated with 6-gingerol under control conditions, and experimental inflammation was induced by tumor necrosis factor-α (TNF-α). Expression of different osteogenic markers and cytokines was analyzed by real-time PCR, Western blotting, and enzyme-linked immunosorbent assay. In addition, alkaline phosphatase (ALP) enzyme activity and biomineralization as markers for differentiation were measured. Treatment with 6-gingerol resulted in insignificant effects on the proliferation rate. 6-Gingerol induced the differentiation of osteoblast-like cells with increased transcription levels of osteogenic markers, upregulated ALP enzyme activity, and enhanced mineralized nodule formation. Stimulation with TNF-α led to enhanced interleukin-6 and nuclear factor-κB expression and downregulated markers of osteoblastic differentiation. 6-Gingerol reduced the degree of inflammation in TNF-α-treated MG-63 cells. In conclusion, 6-gingerol stimulated osteoblast differentiation in normal physiological and inflammatory settings, and therefore, 6-gingerol represents a promising agent for treating osteoporosis or bone inflammation.
Tipo: | Info:eu-repo/semantics/article
Idioma: | Inglês
Identificador: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2015000700637
Editor: | Associação Brasileira de Divulgação Científica
Relação: | 10.1590/1414-431x20154494
Formato: | text/html
Fonte: | Brazilian Journal of Medical and Biological Research  v.48 n.7 2015
Fonte: | info:eu-repo/semantics/openAccess