Data/hora: 01/06/2024 01:16:00

Provedor de dados: 	56	
País: 	Brazil	
Título: 	A phthalide derivative isolated from endophytic fungiPestalotiopsis photiniae induces G1 cell cycle arrest and apoptosis in human HeLa cells	
Autores: 	Chen,C.; Yang,R.L.	
Data: 	2013-08-01	
Ano: 	2013	
Palavras-chave: 	MP [4-(3′; 3′-dimethylallyloxy)-5-methyl-6-methoxyphthalide] p73 Apoptosis.	
Resumo: 	MP [4-(3′,3′-dimethylallyloxy)-5-methyl-6-methoxyphthalide] was obtained from liquid culture of Pestalotiopsis photiniaeisolated from the Chinese Podocarpaceae plant Podocarpus macrophyllus. MP significantly inhibited the proliferation of HeLa tumor cell lines. After treatment with MP, characteristic apoptotic features such as DNA fragmentation and chromatin condensation were observed in DAPI-stained HeLa cells. Flow cytometry showed that MP induced G1 cell cycle arrest and apoptosis in a dose-dependent manner. Western blotting and real-time reverse transcription-polymerase chain reaction were used to investigate protein and mRNA expression. MP caused significant cell cycle arrest by upregulating the cyclin-dependent kinase inhibitor p27KIP1 protein and p21CIP1 mRNA levels in HeLa cells. The expression of p73 protein was increased after treatment with various MP concentrations. mRNA expression of the cell cycle-related genes, p21CIP1, p16INK4a and Gadd45α, was significantly upregulated and mRNA levels demonstrated significantly increased translation ofp73, JunB, FKHR, andBim. The results indicate that MP may be a potential treatment for cervical cancer.	
Tipo: 	Info:eu-repo/semantics/article	
Idioma: 	Inglês	
Identificador: 	http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2013000800643	
Editor: 	Associação Brasileira de Divulgação Científica	
Relação: 	10.1590/1414-431X20132979	
Formato: 	text/html	
Fonte: 	Brazilian Journal of Medical and Biological Research  v.46 n.8 2013	
Fonte: 	info:eu-repo/semantics/openAccess