Data/hora: 06/06/2024 18:12:25

Provedor de dados: | 56
País: | Brazil
Título: | Induction of interleukin-10 by HIV antigens in peripheral mononuclear cells of health care workers after occupational exposure to HIV-1-positive blood
Autores: | Rodrigues,D.S.S.; Brunialti,M.K.C.; Medeiros,E.A.S.; Diaz,R.S.; Turcato,G.; Salomao,R.
Data: | 2002-06-01
Ano: | 2002
Palavras-chave: | HIV-1; Interleukin-10; Needlestick injury; Occupational exposure; Health care worker.
Resumo: | Evaluation of HIV-induced IL-2 production by peripheral blood mononuclear cells (PBMC) and HIV-specific T helper and cytotoxic T lymphocyte (CTL) responses in health care workers (HCW) occupationally exposed to HIV reveals a high rate of response to HIV among non-seroconverters. IL-10 is also known to interfere with HIV infection in vitro. To evaluate the induction of IL-10 by HIV antigens in HCW occupationally exposed to HIV, 18 HCW with percutaneous injury were enrolled in this study, 9 of them exposed to HIV-contaminated blood, and 9 exposed to HIV-negative blood. PBMC were incubated on plates coated with HIV-1 antigens, and IL-10 was measured in supernatants by ELISA. Five of nine HCW exposed to HIV-contaminated blood presented HIV-induced IL-10. Two of nine HCW exposed to HIV-negative source patients also had detectable levels of HIV-induced IL-10, one of them in the sample obtained on the day of accidental exposure. There was a relationship between the type of device involved in injury and IL-10 production. Individuals exposed to hollow needles or scalpels presented HIV-induced IL-10, whereas those exposed to solid needles and to digital puncture did not, suggesting a relationship between infectious load and IL-10. Although occupational exposure to HIV leads to a low rate of seroconversion, these individuals can develop an antigen-specific immune response characterized in our study by induction of IL-10 in PBMC in vitro.
Tipo: | Info:eu-repo/semantics/other
Idioma: | Inglês
Identificador: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2002000600010
Editor: | Associação Brasileira de Divulgação Científica
Relação: | 10.1590/S0100-879X2002000600010
Formato: | text/html
Fonte: | Brazilian Journal of Medical and Biological Research  v.35 n.6 2002
Fonte: | info:eu-repo/semantics/openAccess