Data/hora: 01/06/2024 10:39:00

Provedor de dados: | 56
País: | Brazil
Título: | Multicellular spheroids of bone marrow stromal cells: a three-dimensional in vitro culture system for the study of hematopoietic cell migration
Autores: | Rossi,M.I.D.; Barros,A.P.D.N.; Baptista,L.S.; Garzoni,L.R.; Meirelles,M.N.; Takiya,C.M.; Pascarelli,B.M.O.; Dutra,H.S.; Borojevic,R.
Data: | 2005-10-01
Ano: | 2005
Palavras-chave: | Multicellular spheroid; Three-dimensional culture; Cell migration; Bone marrow.
Resumo: | Cell fate decisions are governed by a complex interplay between cell-autonomous signals and stimuli from the surrounding tissue. In vivo cells are connected to their neighbors and to the extracellular matrix forming a complex three-dimensional (3-D) microenvironment that is not reproduced in conventional in vitro systems. A large body of evidence indicates that mechanical tension applied to the cytoskeleton controls cell proliferation, differentiation and migration, suggesting that 3-D in vitro culture systems that mimic the in vivo situation would reveal biological subtleties. In hematopoietic tissues, the microenvironment plays a crucial role in stem and progenitor cell survival, differentiation, proliferation, and migration. In adults, hematopoiesis takes place inside the bone marrow cavity where hematopoietic cells are intimately associated with a specialized three 3-D scaffold of stromal cell surfaces and extracellular matrix that comprise specific niches. The relationship between hematopoietic cells and their niches is highly dynamic. Under steady-state conditions, hematopoietic cells migrate within the marrow cavity and circulate in the bloodstream. The mechanisms underlying hematopoietic stem/progenitor cell homing and mobilization have been studied in animal models, since conventional two-dimensional (2-D) bone marrow cell cultures do not reproduce the complex 3-D environment. In this review, we will highlight some of the mechanisms controlling hematopoietic cell migration and 3-D culture systems.
Tipo: | Info:eu-repo/semantics/article
Idioma: | Inglês
Identificador: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2005001000002
Editor: | Associação Brasileira de Divulgação Científica
Relação: | 10.1590/S0100-879X2005001000002
Formato: | text/html
Fonte: | Brazilian Journal of Medical and Biological Research  v.38 n.10 2005
Fonte: | info:eu-repo/semantics/openAccess