Data/hora: 01/06/2024 02:46:32

Provedor de dados: | 56
País: | Brazil
Título: | Global microRNA profiles and signaling pathways in the development of cardiac hypertrophy
Autores: | Feng,H.J.; Ouyang,W.; Liu,J.H.; Sun,Y.G.; Hu,R.; Huang,L.H.; Xian,J.L.; Jing,C.F.; Zhou,M.J.
Data: | 2014-05-01
Ano: | 2014
Palavras-chave: | Cardiac hypertrophy; Signaling pathway; Biomarker.
Resumo: | Hypertrophy is a major predictor of progressive heart disease and has an adverse prognosis. MicroRNAs (miRNAs) that accumulate during the course of cardiac hypertrophy may participate in the process. However, the nature of any interaction between a hypertrophy-specific signaling pathway and aberrant expression of miRNAs remains unclear. In this study, Spague Dawley male rats were treated with transverse aortic constriction (TAC) surgery to mimic pathological hypertrophy. Hearts were isolated from TAC and sham operated rats (n=5 for each group at 5, 10, 15, and 20 days after surgery) for miRNA microarray assay. The miRNAs dysexpressed during hypertrophy were further analyzed using a combination of bioinformatics algorithms in order to predict possible targets. Increased expression of the target genes identified in diverse signaling pathways was also analyzed. Two sets of miRNAs were identified, showing different expression patterns during hypertrophy. Bioinformatics analysis suggested the miRNAs may regulate multiple hypertrophy-specific signaling pathways by targeting the member genes and the interaction of miRNA and mRNA might form a network that leads to cardiac hypertrophy. In addition, the multifold changes in several miRNAs suggested that upregulation of rno-miR-331*, rno-miR-3596b, rno-miR-3557-5p and downregulation of rno-miR-10a, miR-221, miR-190, miR-451 could be seen as biomarkers of prognosis in clinical therapy of heart failure. This study described, for the first time, a potential mechanism of cardiac hypertrophy involving multiple signaling pathways that control up- and downregulation of miRNAs. It represents a first step in the systematic discovery of miRNA function in cardiovascular hypertrophy.
Tipo: | Info:eu-repo/semantics/article
Idioma: | Inglês
Identificador: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2014000500361
Editor: | Associação Brasileira de Divulgação Científica
Relação: | 10.1590/1414-431X20142937
Formato: | text/html
Fonte: | Brazilian Journal of Medical and Biological Research  v.47 n.5 2014
Fonte: | info:eu-repo/semantics/openAccess