Data/hora: 02/06/2024 02:54:59

Provedor de dados: | 56
País: | Brazil
Título: | Interleukin-12 and protective immunity to schistosomes
Autores: | Mountford,A.P.; Shires,V.L.; Anderson,S.
Data: | 1998-01-01
Ano: | 1998
Palavras-chave: | Schistosoma; Interleukin-12; Adjuvant; Vaccine; Th1.
Resumo: | The attenuated vaccine against Schistosoma mansoni induces Th1-mediated protective immunity and we have sought to identify a role for IL-12 in this model. Elevated levels of IL-12 (p40 mRNA) were detected in the lymph nodes (LN) and the lungs of vaccinated mice, whilst treatment of vaccinated mice with anti-IL-12 antibodies decreased the ratio of IFN&lt;FONT FACE="Symbol"&gt;g&lt;/font&gt;:IL-4 secreted by in vitro-cultured LN cells. However, there was only marginal abrogation of the level of resistance in these mice. Soluble antigens from the lung-stage of the parasite (SLAP) appeared to be efficient stimulators of IFN&lt;FONT FACE="Symbol"&gt;g&lt;/font&gt; and IL-12 secretion. These antigens when used to immunise mice in conjunction with IL-12 as an adjuvant, elicited a polarised Th1 response with abundant IFN&lt;FONT FACE="Symbol"&gt;g&lt;/font&gt; secretion but no IL-4. This immunisation regime also induced significant protection against reinfection, whereas inoculation of mice with SLAP alone did not. The induction of a dominant Th1 response using SLAP + IL-12 probably operates via IFN&lt;FONT FACE="Symbol"&gt;g&lt;/font&gt; production by natural killer (NK) cells stimulated by IL-12, since in vivo ablation of NK cells using anti-NK1.1 antibody reduced CD4+-dependent IFN&lt;FONT FACE="Symbol"&gt;g&lt;/font&gt; production from cultured LN cells by over 97%. Nevertheless, in mice with a genetic disruption of the IFN&lt;FONT FACE="Symbol"&gt;g&lt;/font&gt; receptor, administration of SLAP + IL-12 induced levels of IFN&lt;FONT FACE="Symbol"&gt;g&lt;/font&gt; equal to those in wild-type mice, thus showing that in this model IL-12 can directly prime T cells independent of IFN&lt;FONT FACE="Symbol"&gt;g&lt;/font&gt;. Clearly, IL-12 has a critical role in protective immunity to schistosomes and it may aid the development of an effective vaccine against this disease
Tipo: | Info:eu-repo/semantics/article
Idioma: | Inglês
Identificador: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X1998000100023
Editor: | Associação Brasileira de Divulgação Científica
Relação: | 10.1590/S0100-879X1998000100023
Formato: | text/html
Fonte: | Brazilian Journal of Medical and Biological Research  v.31 n.1 1998
Fonte: | info:eu-repo/semantics/openAccess