Data/hora: 06/06/2024 09:36:01

Provedor de dados: | 56
País: | Brazil
Título: | Myostatin promotes distinct responses on protein metabolism of skeletal and cardiac muscle fibers of rodents
Autores: | Manfredi,L.H.; Paula-Gomes,S.; Zanon,N.M.; Kettelhut,I.C.
Data: | 2017-01-01
Ano: | 2017
Palavras-chave: | Myostatin; Skeletal muscle; Cardiomyocytes; Protein degradation; Protein synthesis.
Resumo: | Myostatin is a novel negative regulator of skeletal muscle mass. Myostatin expression is also found in heart in a much less extent, but it can be upregulated in pathological conditions, such as heart failure. Myostatin may be involved in inhibiting protein synthesis and/or increasing protein degradation in skeletal and cardiac muscles. Herein, we used cell cultures and isolated muscles from rats to determine protein degradation and synthesis. Muscles incubated with myostatin exhibited an increase in proteolysis with an increase of Atrogin-1, MuRF1 and LC3 genes. Extensor digitorum longus muscles and C2C12 myotubes exhibited a reduction in protein turnover. Cardiomyocytes showed an increase in proteolysis by activating autophagy and the ubiquitin proteasome system, and a decrease in protein synthesis by decreasing P70S6K. The effect of myostatin on protein metabolism is related to fiber type composition, which may be associated to the extent of atrophy mediated effect of myostatin on muscle.
Tipo: | Info:eu-repo/semantics/article
Idioma: | Inglês
Identificador: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2017001200604
Editor: | Associação Brasileira de Divulgação Científica
Relação: | 10.1590/1414-431x20176733
Formato: | text/html
Fonte: | Brazilian Journal of Medical and Biological Research  v.50 n.12 2017
Fonte: | info:eu-repo/semantics/openAccess