Data/hora: 07/06/2024 06:35:27

Provedor de dados: | 56
País: | Brazil
Título: | Thiol-independent activity of a cholesterol-binding enterohemolysin produced by enteropathogenic Escherichia coli
Autores: | Figueirêdo,P.M.S.; Catani,C.F.; Yano,T.
Data: | 2003-11-01
Ano: | 2003
Palavras-chave: | Escherichia coli; Enterohemolysin; Thiol-independent activity; Cholesterol binding.
Resumo: | Enterohemolysin produced by Escherichia coli associated with infant diarrhea showed characteristics similar to those of thiol-activated hemolysins produced by Gram-positive bacteria, including inactivation by cholesterol, lytic activity towards eukaryotic cells and thermoinstability. However, enterohemolysin activity was not inactivated by oxidation or by SH group-blocking agents (1 mM HgCl2, 1 mM iodoacetic acid) and the hemolysin (100 µg/ml) was not lethal to mice, in contrast to the lethality of the thiol-activated hemolysin family to animals. Earlier reports showed that intravenous injection of partially purified streptolysin O preparations (0.2 µg) was rapidly lethal to mice. These results suggest that E. coli enterohemolysin is not a thiol-activated hemolysin, despite its ability to bind cholesterol, probably due to the absence of free thiol-group(s) that characterize the active form of the thiol-activated hemolysin molecule.
Tipo: | Info:eu-repo/semantics/other
Idioma: | Inglês
Identificador: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2003001100008
Editor: | Associação Brasileira de Divulgação Científica
Relação: | 10.1590/S0100-879X2003001100008
Formato: | text/html
Fonte: | Brazilian Journal of Medical and Biological Research  v.36 n.11 2003
Fonte: | info:eu-repo/semantics/openAccess