Data/hora: 11/06/2024 10:58:51

Provedor de dados: | 56
País: | Brazil
Título: | Apoptosis of sensory neurons and satellite cells after sciatic nerve transection in C57BL/6J mice
Autores: | Oliveira,A.L.R.
Data: | 2001-03-01
Ano: | 2001
Palavras-chave: | TUNEL; Apoptosis; Axotomy; Neuronal death; Satellite cells; Isogenic mice.
Resumo: | The rate of axonal regeneration, after sciatic nerve lesion in adult C57BL/6J mice, is reduced when compared to other isogenic strains. It was observed that such low regeneration was not due just to a delay, since neuronal death was observed. Two general mechanisms of cell death, apoptosis and necrosis, may be involved. By using the terminal deoxynucleotidyl transferase dUTP nick end labeling (TUNEL) technique, we demonstrated that a large number of sensory neurons, as well as satellite cells found in the dorsal root ganglia, were intensely labeled, thus indicating that apoptotic mechanisms were involved in the death process. Although almost no labeled neurons or satellite cells were observed one week after transection, a more than ten-fold increase in TUNEL labeling was detected after two weeks. The results obtained with the C57BL/6J strain were compared with those of the A/J strain, which has a much higher peripheral nerve regeneration potential. In A/J mice, almost no labeling of sensory neurons or satellite cells was observed after one or two weeks, indicating the absence of neuronal loss. Our data confirm previous observations that approximately 40% of C57BL/6J sensory neurons die after sciatic nerve transection, and indicate that apoptotic events are involved. Also, our observations reinforce the hypothesis that the low rate of axonal regeneration occurring in C57BL/6J mice may be the result of a mismatch in the timing of the neurons need for neurotrophic substances, and production of the latter by non-neuronal cells in the distal stump.
Tipo: | Info:eu-repo/semantics/other
Idioma: | Inglês
Identificador: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2001000300012
Editor: | Associação Brasileira de Divulgação Científica
Relação: | 10.1590/S0100-879X2001000300012
Formato: | text/html
Fonte: | Brazilian Journal of Medical and Biological Research  v.34 n.3 2001
Fonte: | info:eu-repo/semantics/openAccess