Data/hora: 11/06/2024 08:06:09

Provedor de dados: | 56
País: | Brazil
Título: | Metabolism of diglycine and triglycine by non-filtering kidneys
Data: | 1997-02-01
Ano: | 1997
Palavras-chave: | Non-filtering kidney; Dipeptide; Tripeptide; Metabolism; Peptidases.
Resumo: | We have studied the metabolism of diglycine and triglycine in the isolated non-filtering rat kidney. Kidneys from adult male Wistar Kyoto rats weighing 250-350 g were perfused with Krebs-Henseleit solution containing either 1 mM diglycine or triglycine. The analysis of the peptide residues and their components was performed using an amino acid microanalyzer utilizing ion exchange chromatography. Diglycine was degraded to a final concentration of 0.09 mM after 120 min (91%); this degradation occurred predominantly during the first hour, with a 56% reduction of the initial concentration. The metabolism of triglycine occurred similarly, with a final concentration of 0.18 mM (82%); during the first hour there was a 67% reduction of the initial concentration of the tripeptide. Both peptides produced glycine in increasing concentrations, but there was a slightly lower recovery of glycine, suggesting its utilization by the kidney as fuel. The hydrolysis of triglycine also produced diglycine, which was also hydrolyzed to glycine. The results of the present study show the existence of functional endothelial or contraluminal membrane peptidases which may be important during parenteral nutrition.
Tipo: | Info:eu-repo/semantics/other
Idioma: | Inglês
Identificador: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X1997000200005
Editor: | Associação Brasileira de Divulgação Científica
Relação: | 10.1590/S0100-879X1997000200005
Formato: | text/html
Fonte: | Brazilian Journal of Medical and Biological Research  v.30 n.2 1997
Fonte: | info:eu-repo/semantics/openAccess