Data/hora: 06/06/2024 11:42:31

Provedor de dados: | 56
País: | Brazil
Título: | Adiponectin inhibits oxidization-induced differentiation of T helper cells through inhibiting costimulatory CD40 and CD80
Autores: | Xiao,Y.; Deng,T.; Shang,Z.; Wang,D.
Data: | 2017-01-01
Ano: | 2017
Palavras-chave: | Adiponectin; T helper cells; HLA-DR; CD80; CD40.
Resumo: | Adiponectin is a multifunctional adipokine that has several oligomeric forms in the blood stream, which broadly regulates innate and acquired immunity. Therefore, in this study, we aimed to observe the differentiation of T helper (Th) cells and expression of costimulatory signaling molecules affected by adiponectin. The mRNA and protein expression levels of adiponectin and its receptors in oxidized low density lipoprotein cholesterol-treated endothelial cells were assayed by real time PCR and immunofluorescence. The endothelial cells were then treated with adiponectin with or without adipoR1 or adipoR2 siRNA and co-cultured with T lymphocytes. The distribution of Th1, Th2 and Th17 subsets were assayed by flow cytometry. The effects of adiponectin on costimulatory signaling molecules HLA-DR, CD80, CD86 and CD 40 was also assayed by flow cytometry. The results showed that endothelial cells expressed adiponectin and its receptor adipoR1 and adipoR2, but not T-cadherin. Adiponectin suppressed Th1 and Th17 differentiation through adipoR1 receptor, contributed to the inhibition of CD80 and CD40, and inhibited differentiation of Th1 and Th17 by inhibiting antigen presenting action.
Tipo: | Info:eu-repo/semantics/article
Idioma: | Inglês
Identificador: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2017000600606
Editor: | Associação Brasileira de Divulgação Científica
Relação: | 10.1590/1414-431x20176227
Formato: | text/html
Fonte: | Brazilian Journal of Medical and Biological Research  v.50 n.6 2017
Fonte: | info:eu-repo/semantics/openAccess