Data/hora: 01/06/2024 04:40:24

Provedor de dados: 	56	
País: 	Brazil	
Título: 	Fingerprinting of cell lines by directed amplification of minisatellite-region DNA (DAMD)	
Autores: 	Silva,L.M.; Montes de Oca,H.; Diniz,C.R.; Fortes-Dias,C.L.	
Data: 	2001-11-01	
Ano: 	2001	
Palavras-chave: 	DNA fingerprinting; DAMD; Cell line; Cell culture.	
Resumo: 	The development of in vitro propagation of cells has been an extraordinary technical advance for several biological studies. The correct identification of the cell line used, however, is crucial, as a mistaken identity or the presence of another contaminating cell may lead to invalid and/or erroneous conclusions. We report here the application of a DNA fingerprinting procedure (directed amplification of minisatellite-region DNA), developed by Heath et al. [Nucleic Acids Research (1993) 21: 5782-5785], to the characterization of cell lines. Genomic DNA of cells in culture was extracted and amplified by PCR in the presence of VNTR core sequences, and the amplicons were separated by agarose gel electrophoresis. After image capture with a digital camera, the banding profiles obtained were analyzed using a software (AnaGel) specially developed for the storage and analysis of electrophoretic fingerprints. The fingerprints are useful for construction of a data base for identification of cell lines by comparison to reference profiles as well as comparison of similar lines from different sources and periodic follow-up of cells in culture.	
Tipo: 	Info:eu-repo/semantics/article	
Idioma: 	Inglês	
Identificador: 	http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2001001100005	
Editor: 	Associação Brasileira de Divulgação Científica	
Relação: 	10.1590/S0100-879X2001001100005	
Formato: 	text/html	
Fonte: 	Brazilian Journal of Medical and Biological Research  v.34 n.11 2001	
Fonte: 	info:eu-repo/semantics/openAccess