Data/hora: 05/06/2024 14:28:44

Provedor de dados: 	56	
País: 	Brazil	
Título: 	Proteomic inventory of myocardial proteins from patients with chronic Chagas' cardiomyopathy	
Autores: 	Teixeira,P.C.; Iwai,L.K.; Kuramoto,A.C.K.; Honorato,R.; Fiorelli,A.; Stolf,N.; Kalil,J.; Cunha-Neto,E.	
Data: 	2006-12-01	
Ano: 	2006	
Palavras-chave: 	Chagas' disease; Cardiomyopathy; Proteomic analysis; Two-dimensional electrophoresis; MALDI-ToF.	
Resumo: 	Chronic Chagas' disease cardiomyopathy (CCC) is an often fatal outcome of Trypanosoma cruzi infection, with a poorer prognosis than other cardiomyopathies. CCC is refractory to heart failure treatments, and is the major indication of heart transplantation in Latin America. A diffuse myocarditis, plus intense myocardial hypertrophy, damage and fibrosis, in the presence of very few T. cruzi forms, are the histopathological hallmarks of CCC. To gain a better understanding of the pathophysiology of CCC, we analyzed the protein profile in the affected CCC myocardium. Homogenates from left ventricular myocardial samples of end-stage CCC hearts explanted during heart transplantation were subjected to two-dimensional electrophoresis with Coomassie blue staining; protein identification was performed by MALDI-ToF mass spectrometry and peptide mass fingerprinting. The identification of selected proteins was confirmed by immunoblotting. We demonstrated that 246 proteins matched in gels from two CCC patients. They corresponded to 112 distinct proteins. Along with structural/contractile and metabolism proteins, we also identified proteins involved in apoptosis (caspase 8, caspase 2), immune system (T cell receptor ß chain, granzyme A, HLA class I) and stress processes (heat shock proteins, superoxide dismutases, and other oxidative stress proteins). Proteins involved in cell signaling and transcriptional factors were also identified. The identification of caspases and oxidative stress proteins suggests the occurrence of active apoptosis and significant oxidative stress in CCC myocardium. These results generated an inventory of myocardial proteins in CCC that should contribute to the generation of hypothesis-driven experiments designed on the basis of the classes of proteins identified here.	
Tipo: 	Info:eu-repo/semantics/article	
Idioma: 	Inglês	
Identificador: 	http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2006001200005	
Editor: 	Associação Brasileira de Divulgação Científica	
Relação: 	10.1590/S0100-879X2006005000050	
Formato: 	text/html	
Fonte: 	Brazilian Journal of Medical and Biological Research  v.39 n.12 2006	
Fonte: 	info:eu-repo/semantics/openAccess