Data/hora: 07/06/2024 00:54:01

Provedor de dados: | 56
País: | Brazil
Título: | Characterization of six rat strains (Rattus norvegicus) by mitochondrial DNA restriction fragment length polymorphism
Autores: | Hilsdorf,A.W.; Krieger,J.E.
Data: | 1999-03-01
Ano: | 1999
Palavras-chave: | Restriction fragment length polymorphism (RFLP); Rattus norvegicus; MtDNA; Laboratory rats; Genetic monitoring.
Resumo: | Restriction fragment length polymorphism (RFLP) was used to examine the extent of mtDNA polymorphism among six strains of rats (Rattus norvegicus) - Wistar, Wistar Munich, Brown Norway, Wistar Kyoto, SHR and SHR-SP. A survey of 26 restriction enzymes has revealed a low level of genetic divergence among strains. The sites of cleavage by EcoRI, NcoI and XmnI were shown to be polymorphic. The use of these three enzymes allows the 6 strains to be classified into 4 haplotypes and identifies specific markers for each one. The percentage of sequence divergence among all pairs of haplotypes ranged from 0.035 to 0.33%, which is the result of a severe population constriction undergone by the strains. These haplotypes are easily demonstrable and therefore RFLP analysis can be employed for genetic monitoring of rats within animal facilities or among different laboratories.
Tipo: | Info:eu-repo/semantics/article
Idioma: | Inglês
Identificador: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X1999000300003
Editor: | Associação Brasileira de Divulgação Científica
Relação: | 10.1590/S0100-879X1999000300003
Formato: | text/html
Fonte: | Brazilian Journal of Medical and Biological Research  v.32 n.3 1999
Fonte: | info:eu-repo/semantics/openAccess