Data/hora: 05/06/2024 09:14:07

Provedor de dados: | 56
País: | Brazil
Título: | The role of T cell subsets and cytokines in the regulation of intracellular bacterial infection
Autores: | Oliveira,S.C.; Harms,J.S.; Rech,E.L.; Rodarte,R.S.; Bocca,A.L.; Goes,A.M.; Splitter,G.A.
Data: | 1998-01-01
Ano: | 1998
Palavras-chave: | T cell subsets; Brucella abortus; Knockout mice; Cytokines; Intracellular pathogens; Genetic immunization.
Resumo: | Cellular immune responses are a critical part of the host's defense against intracellular bacterial infections. Immunity to Brucella abortus crucially depends on antigen-specific T cell-mediated activation of macrophages, which are the major effectors of cell-mediated killing of this organism. T lymphocytes that proliferate in response to B. abortus were characterized for phenotype and cytokine activity. Human, murine, and bovine T lymphocytes exhibited a type 1 cytokine profile, suggesting an analogous immune response in these different hosts. In vivo protection afforded by a particular cell type is dependent on the antigen presented and the mechanism of antigen presentation. Studies using MHC class I and class II knockout mice infected with B. abortus have demonstrated that protective immunity to brucellosis is especially dependent on CD8+ T cells. To target MHC class I presentation we transfected ex vivo a murine macrophage cell line with B. abortus genes and adoptively transferred them to BALB/c mice. These transgenic macrophage clones induced partial protection in mice against experimental brucellosis. Knowing the cells required for protection, vaccines can be designed to activate the protective T cell subset. Lastly, as a new strategy for priming a specific class I-restricted T cell response in vivo, we used genetic immunization by particle bombardment-mediated gene transfer
Tipo: | Info:eu-repo/semantics/article
Idioma: | Inglês
Identificador: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X1998000100010
Editor: | Associação Brasileira de Divulgação Científica
Relação: | 10.1590/S0100-879X1998000100010
Formato: | text/html
Fonte: | Brazilian Journal of Medical and Biological Research  v.31 n.1 1998
Fonte: | info:eu-repo/semantics/openAccess