Data/hora: 10/06/2024 09:03:53

Provedor de dados: | 56
País: | Brazil
Título: | Partial sequence and toxic effects of granulitoxin, a neurotoxic peptide from the sea anemone Bunodosoma granulifera
Autores: | Santana,A.N.C.; Leite,A.B.; França,M.S.F.; França,L.; Vale,O.C.; Cunha,R.B.; Ricart,C.A.O.; Sousa,M.V.; Carvalho,K.M.
Data: | 1998-10-01
Ano: | 1998
Palavras-chave: | Bunodosoma granulifera; Sea anemones; Toxic peptides.
Resumo: | A neurotoxic peptide, granulitoxin (GRX), was isolated from the sea anemone Bunodosoma granulifera. The N-terminal amino acid sequence of GRX is AKTGILDSDGPTVAGNSLSGT and its molecular mass is 4958 Da by electrospray mass spectrometry. This sequence presents a partial degree of homology with other toxins from sea anemones such as Bunodosoma caissarum, Anthopleura fuscoviridis and Anemonia sulcata. However, important differences were found: the first six amino acids of the sequence are different, Arg-14 was replaced by Ala and no cysteine residues were present in the partial sequence, while two cysteine residues were present in the first 21 amino acids of other toxins described above. Purified GRX injected ip (800 µg/kg) into mice produced severe neurotoxic effects such as circular movements, aggressive behavior, dyspnea, tonic-clonic convulsion and death. The 2-h LD50 of GRX was 400 ± 83 µg/kg.
Tipo: | Info:eu-repo/semantics/other
Idioma: | Inglês
Identificador: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X1998001000015
Editor: | Associação Brasileira de Divulgação Científica
Relação: | 10.1590/S0100-879X1998001000015
Formato: | text/html
Fonte: | Brazilian Journal of Medical and Biological Research  v.31 n.10 1998
Fonte: | info:eu-repo/semantics/openAccess