Data/hora: 05/06/2024 18:00:04

Provedor de dados: 	56	
País: 	Brazil	
Título: 	Effect of estradiol and bisphenol A on human hepatoblastoma cell viability and telomerase activity	
Autores: 	Xu,B.L.; Zhao,Q.Z.; Gao,X.Y.; Hou,G.J.	
Data: 	2015-11-01	
Ano: 	2015	
Palavras-chave: 	Estradiol; Bisphenol A; HepG2; Telomerase activity.	
Resumo: 	Sex hormones from environmental and physiological sources might play a major role in the pathogenesis of hepatoblastoma in children. This study investigated the effects of estradiol and bisphenol A on the proliferation and telomerase activity of human hepatoblastoma HepG2 cells. The cells were divided into 6 treatment groups: control, bisphenol A, estradiol, anti-estrogen ICI 182,780 (hereinafter ICI), bisphenol A+ICI, and estradiol+ICI. Cell proliferation was measured based on average absorbance using the Cell Counting-8 assay. The cell cycle distribution and apoptotic index were determined by flow cytometry. Telomerase activity was detected by polymerase chain reaction and a telomeric repeat amplification protocol assay. A higher cell density was observed in bisphenol A (P&lt;0.01) and estradiol (P&lt;0.05) groups compared with the control group. Cell numbers in S and G2/M phases after treatment for 48 h were higher (P&lt;0.05), while the apoptotic index was lower (P&lt;0.05) and telomerase activities at 48 and 72 h (P&lt;0.05) were higher in these groups than in the control group. The cell density was also higher in bisphenol A+ICI (P&lt;0.01) and estradiol+ICI (P&lt;0.05) groups compared with the ICI group. Furthermore, cell numbers were increased in S and G2/M phases (P&lt;0.05), while the apoptotic index was lower (P&lt;0.05) and telomerase activities at 48 and 72 h were higher (P&lt;0.05) in these groups than in the ICI group. Therefore, bisphenol A and estradiol promote HepG2 cell proliferation in vitro by inhibition of apoptosis and stimulation of telomerase activity via an estrogen receptor-dependent pathway.	
Tipo: 	Info:eu-repo/semantics/article	
Idioma: 	Inglês	
Identificador: 	http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2015001101004	
Editor: 	Associação Brasileira de Divulgação Científica	
Relação: 	10.1590/1414-431x20154400	
Formato: 	text/html	
Fonte: 	Brazilian Journal of Medical and Biological Research  v.48 n.11 2015	
Fonte: 	info:eu-repo/semantics/openAccess