Data/hora: 09/06/2024 16:57:59

Provedor de dados: 	56	
País: 	Brazil	
Título: 	Antisense mRNA for NPY-Y1 receptor in the medial preoptic area increases prolactin secretion	
Autores: 	Silveira,N.A.; Franci,C.R.	
Data: 	1999-09-01	
Ano: 	1999	
Palavras-chave: 	Neuropeptide Y; Medial preoptic area; Neuropeptide Y-Y1 receptor; Prolactin; MRNA for neuropeptide; Y-Y1 receptor; Luteinizing hormone; Follicle-stimulating hormone.	
Resumo: 	We investigated the participation of neuropeptide Y-Y1 receptors within the medial preoptic area in luteinizing hormone, follicle-stimulating hormone and prolactin release. Four bilateral microinjections of sense (control) or antisense 18-base oligonucleotides of messenger ribonucleic acid (mRNA) (250 ng) corresponding to the NH2-terminus of the neuropeptide Y1 receptor were performed at 12-h intervals for two days into the medial preoptic area of ovariectomized Wistar rats (N = 16), weighing 180 to 200 g, treated with estrogen (50 µg) and progesterone (25 mg) two days before the experiments between 8.00 and 10:00 a.m. Blockade of Y1 receptor synthesis in the medial preoptic area by the antisense mRNA did not change plasma luteinizing hormone or follicle-stimulating hormone but did increase prolactin from 19.6 ± 5.9 ng/ml in the sense group to 52.9 ± 9.6 ng/ml in the antisense group. The plasma hormones were measured by radioimmunoassay and the values are reported as mean ± SEM. These data suggest that endogenous neuropeptide Y in the medial preoptic area has an inhibitory action on prolactin secretion through Y1 receptors.	
Tipo: 	Info:eu-repo/semantics/article	
Idioma: 	Inglês	
Identificador: 	http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X1999000900016	
Editor: 	Associação Brasileira de Divulgação Científica	
Relação: 	10.1590/S0100-879X1999000900016	
Formato: 	text/html	
Fonte: 	Brazilian Journal of Medical and Biological Research  v.32 n.9 1999	
Fonte: 	info:eu-repo/semantics/openAccess