Data/hora: 02/06/2024 10:32:02

Provedor de dados: | 56
País: | Brazil
Título: | Detection of multiple mycoplasma infection in cell cultures by PCR
Autores: | Timenetsky,J.; Santos,L.M.; Buzinhani,M.; Mettifogo,E.
Data: | 2006-07-01
Ano: | 2006
Palavras-chave: | Mycoplasma; Cell culture; Multiple infection; Mycoplasma detection.
Resumo: | A total of 301 cell cultures from 15 laboratories were monitored for mycoplasma (Mollicutes) using PCR and culture methodology. The infection was detected in the cell culture collection of 12 laboratories. PCR for Mollicutes detected these bacteria in 93 (30.9%) samples. Although the infection was confirmed by culture for 69 (22.9%) samples, PCR with generic primers did not detect the infection in five (5.4%). Mycoplasma species were identified with specific primers in 91 (30.2%) of the 98 samples (32.6%) considered to be infected. Mycoplasma hyorhinis was detected in 63.3% of the infected samples, M. arginini in 59.2%, Acholeplasma laidlawii in 20.4%, M. fermentans in 14.3%, M. orale in 11.2%, and M. salivarium in 8.2%. Sixty (61.2%) samples were co-infected with more than one mycoplasma species. M. hyorhinis and M. arginini were the microorganisms most frequently found in combination, having been detected in 30 (30.6%) samples and other associations including up to four species were detected in 30 other samples. Failure of the treatments used to eliminate mycoplasmas from cell cultures might be explained by the occurrence of these multiple infections. The present results indicate that the sharing of non-certified cells among laboratories may disseminate mycoplasma in cell cultures.
Tipo: | Info:eu-repo/semantics/article
Idioma: | Inglês
Identificador: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2006000700009
Editor: | Associação Brasileira de Divulgação Científica
Relação: | 10.1590/S0100-879X2006000700009
Formato: | text/html
Fonte: | Brazilian Journal of Medical and Biological Research  v.39 n.7 2006
Fonte: | info:eu-repo/semantics/openAccess