Data/hora: 03/06/2024 06:24:16

Provedor de dados: 	56	
País: 	Brazil	
Título: 	Low-intensity red and infrared laser effects at high fluences on Escherichia coli cultures	
Autores: 	Barboza,L.L.; Campos,V.M.A.; Magalhães,L.A.G.; Paoli,F.; Fonseca,A.S.	
Data: 	2015-10-01	
Ano: 	2015	
Palavras-chave: 	DNA; Escherichia coli; Filamentation; Laser.	
Resumo: 	Semiconductor laser devices are readily available and practical radiation sources providing wavelength tenability and high monochromaticity. Low-intensity red and near-infrared lasers are considered safe for use in clinical applications. However, adverse effects can occur via free radical generation, and the biological effects of these lasers from unusually high fluences or high doses have not yet been evaluated. Here, we evaluated the survival, filamentation induction and morphology of Escherichia coli cells deficient in repair of oxidative DNA lesions when exposed to low-intensity red and infrared lasers at unusually high fluences. Cultures of wild-type (AB1157), endonuclease III-deficient (JW1625-1), and endonuclease IV-deficient (JW2146-1) E. coli, in exponential and stationary growth phases, were exposed to red and infrared lasers (0, 250, 500, and 1000 J/cm2) to evaluate their survival rates, filamentation phenotype induction and cell morphologies. The results showed that low-intensity red and infrared lasers at high fluences are lethal, induce a filamentation phenotype, and alter the morphology of the E. coli cells. Low-intensity red and infrared lasers have potential to induce adverse effects on cells, whether used at unusually high fluences, or at high doses. Hence, there is a need to reinforce the importance of accurate dosimetry in therapeutic protocols.	
Tipo: 	Info:eu-repo/semantics/article	
Idioma: 	Inglês	
Identificador: 	http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2015001000013	
Editor: 	Associação Brasileira de Divulgação Científica	
Relação: 	10.1590/1414-431x20154460	
Formato: 	text/html	
Fonte: 	Brazilian Journal of Medical and Biological Research  v.48 n.10 2015	
Fonte: 	info:eu-repo/semantics/openAccess