Data/hora: 11/06/2024 16:45:22

Provedor de dados: | 56
País: | Brazil
Título: | Pitfalls in the assessment of murine atherosclerosis
Autores: | Catanozi,S.; Rocha,J.C.; Passarelli,M.; Chiquito,F.C.; Quintão,E.C.R.; Nakandakare,E.R.
Data: | 2009-06-01
Ano: | 2009
Palavras-chave: | Atherosclerosis; Animal models; Disease models; Aorta; Pathology.
Resumo: | This review provides examples of the fact that different procedures for the measurement of atherosclerosis in mice may lead to interpretation of the extent of atherosclerosis having markedly different biological and clinical significance for humans: 1) aortic cholesterol measurement is highly sensitive for the detection of early and advanced atherosclerosis lesions, but misses the identification of the location and complexity of these lesions that are so critical for humans; 2) the histological analysis of the aortic root lesions in simvastatin-treated and control mice reveals similar lesion morphology in spite of the remarkable simvastatin-induced reduction of the aortic cholesteryl ester content; 3) in histological analyses, chemical fixation and inclusion may extract the tissue fat and also shrink and distort tissue structures. Thus, the method may be less sensitive for the detection of slight differences among the experimental groups, unless a more suitable procedure employing physical fixation with histological sample freezing using optimal cutting temperature and liquid nitrogen is employed. Thus, when measuring experimental atherosclerosis in mice, investigators should be aware of several previously unreported pitfalls regarding the extent, location and complexity of the arterial lesion that may not be suitable for extrapolation to human pathology.
Tipo: | Info:eu-repo/semantics/article
Idioma: | Inglês
Identificador: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2009000600001
Editor: | Associação Brasileira de Divulgação Científica
Relação: | 10.1590/S0100-879X2009000600001
Formato: | text/html
Fonte: | Brazilian Journal of Medical and Biological Research  v.42 n.6 2009
Fonte: | info:eu-repo/semantics/openAccess