Data/hora: 06/06/2024 16:46:44

Provedor de dados: | 56
País: | Brazil
Título: | Proteoliposomes as matrix vesicles' biomimetics to study the initiation of skeletal mineralization
Autores: | Simão,A.M.S.; Yadav,M.C.; Ciancaglini,P.; Millán,J.L.
Data: | 2010-03-01
Ano: | 2010
Palavras-chave: | Alkaline phosphatase; Biomineralization; Calcification; Lipids; Pyrophosphate; ATP.
Resumo: | During the process of endochondral bone formation, chondrocytes and osteoblasts mineralize their extracellular matrix by promoting the formation of hydroxyapatite (HA) seed crystals in the sheltered interior of membrane-limited matrix vesicles (MVs). Ion transporters control the availability of phosphate and calcium needed for HA deposition. The lipidic microenvironment in which MV-associated enzymes and transporters function plays a crucial physiological role and must be taken into account when attempting to elucidate their interplay during the initiation of biomineralization. In this short mini-review, we discuss the potential use of proteoliposome systems as chondrocyte- and osteoblast-derived MVs biomimetics, as a means of reconstituting a phospholipid microenvironment in a manner that recapitulates the native functional MV microenvironment. Such a system can be used to elucidate the interplay of MV enzymes during catalysis of biomineralization substrates and in modulating in vitro calcification. As such, the enzymatic defects associated with disease-causing mutations in MV enzymes could be studied in an artificial vesicular environment that better mimics their in vivo biological milieu. These artificial systems could also be used for the screening of small molecule compounds able to modulate the activity of MV enzymes for potential therapeutic uses. Such a nanovesicular system could also prove useful for the repair/treatment of craniofacial and other skeletal defects and to facilitate the mineralization of titanium-based tooth implants.
Tipo: | Info:eu-repo/semantics/article
Idioma: | Inglês
Identificador: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2010000300003
Editor: | Associação Brasileira de Divulgação Científica
Relação: | 10.1590/S0100-879X2010007500008
Formato: | text/html
Fonte: | Brazilian Journal of Medical and Biological Research  v.43 n.3 2010
Fonte: | info:eu-repo/semantics/openAccess