Data/hora: 03/06/2024 10:05:20

Provedor de dados: | 56
País: | Brazil
Título: | Characterization of the mucosal and systemic immune response induced by Cry1Ac protein from Bacillus thuringiensis HD 73 in mice
Autores: | Vázquez-Padrón,R.I.; Moreno-Fierros,L.; Neri-Bazán,L.; Martínez-Gil,A.F.; de-la-Riva,G.A.; López-Revilla,R.
Data: | 2000-02-01
Ano: | 2000
Palavras-chave: | Cry proteins; Bacillus thuringiensis; Mucosal immunology.
Resumo: | The present paper describes important features of the immune response induced by the Cry1Ac protein from Bacillus thuringiensis in mice. The kinetics of induction of serum and mucosal antibodies showed an immediate production of anti-Cry1Ac IgM and IgG antibodies in serum after the first immunization with the protoxin by either the intraperitoneal or intragastric route. The antibody fraction in serum and intestinal fluids consisted mainly of IgG1. In addition, plasma cells producing anti-Cry1Ac IgG antibodies in Peyer's patches were observed using the solid-phase enzyme-linked immunospot (ELISPOT). Cry1Ac toxin administration induced a strong immune response in serum but in the small intestinal fluids only anti-Cry1Ac IgA antibodies were detected. The data obtained in the present study confirm that the Cry1Ac protoxin is a potent immunogen able to induce a specific immune response in the mucosal tissue, which has not been observed in response to most other proteins.
Tipo: | Info:eu-repo/semantics/article
Idioma: | Inglês
Identificador: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2000000200002
Editor: | Associação Brasileira de Divulgação Científica
Relação: | 10.1590/S0100-879X2000000200002
Formato: | text/html
Fonte: | Brazilian Journal of Medical and Biological Research  v.33 n.2 2000
Fonte: | info:eu-repo/semantics/openAccess