Data/hora: 10/06/2024 11:23:46

Provedor de dados: | 56
País: | Brazil
Título: | Tumor necrosis factor alpha increases epithelial barrier permeability by disrupting tight junctions in Caco-2 cells
Autores: | Cui,W.; Li,L.X.; Sun,C.M.; Wen,Y.; Zhou,Y.; Dong,Y.L.; Liu,P.
Data: | 2010-04-01
Ano: | 2010
Palavras-chave: | TNF-α; Intestinal epithelial barrier; Occludin; Caco-2 cells; Transepithelial permeability.
Resumo: | The objectives of this study were to determine the effect of tumor necrosis factor alpha (TNF-α) on intestinal epithelial cell permeability and the expression of tight junction proteins. Caco-2 cells were plated onto Transwell® microporous filters and treated with TNF-α (10 or 100 ng/mL) for 0, 4, 8, 16, or 24 h. The transepithelial electrical resistance and the mucosal-to-serosal flux rates of the established paracellular marker Lucifer yellow were measured in filter-grown monolayers of Caco-2 intestinal cells. The localization and expression of the tight junction protein occludin were detected by immunofluorescence and Western blot analysis, respectively. SYBR-Green-based real-time PCR was used to measure the expression of occludin mRNA. TNF-α treatment produced concentration- and time-dependent decreases in Caco-2 transepithelial resistance and increases in transepithelial permeability to the paracellular marker Lucifer yellow. Western blot results indicated that TNF-α decreased the expression of phosphorylated occludin in detergent-insoluble fractions but did not affect the expression of non-phosphorylated occludin protein. Real-time RT-PCR data showed that TNF-α did not affect the expression of occludin mRNA. Taken together, our data demonstrate that TNF-α increases Caco-2 monolayer permeability, decreases occludin protein expression and disturbs intercellular junctions.
Tipo: | Info:eu-repo/semantics/article
Idioma: | Inglês
Identificador: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2010000400002
Editor: | Associação Brasileira de Divulgação Científica
Relação: | 10.1590/S0100-879X2010007500020
Formato: | text/html
Fonte: | Brazilian Journal of Medical and Biological Research  v.43 n.4 2010
Fonte: | info:eu-repo/semantics/openAccess