Data/hora: 05/06/2024 06:44:24

Provedor de dados: | 56
País: | Brazil
Título: | A gasometric method to determine erythrocyte catalase activity
Autores: | Siqueira,A.J.S.; Remião,J.O.; Azevedo,A.M.P.; Azambuja,C.R.J.
Data: | 1999-09-01
Ano: | 1999
Palavras-chave: | Erythrocyte; Catalase assay; Gasometry.
Resumo: | We describe a new gasometric method to determine erythrocyte catalase activity by the measurement of the volume of oxygen produced as a result of hydrogen peroxide decomposition in a system where enzyme and substrate are separated in a special reaction test tube connected to a manometer and the reagents are mixed with a motor-driven stirrer. The position of the reagents in the test tube permits the continuous measurement of oxygen evolution from the time of mixing, without the need to stop the reaction by the addition of acid after each incubation time. The enzyme activity is reported as KHb, i.e., mg hydrogen peroxide decomposed per second per gram of hemoglobin (s-1 g Hb-1). The value obtained for catalase activity in 28 samples of hemolyzed human blood was 94.4 ± 6.17 mg H2O2 s-1 g Hb-1. The results obtained were precise and consistent, indicating that this rapid, simple and inexpensive method could be useful for research and routine work.
Tipo: | Info:eu-repo/semantics/article
Idioma: | Inglês
Identificador: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X1999000900006
Editor: | Associação Brasileira de Divulgação Científica
Relação: | 10.1590/S0100-879X1999000900006
Formato: | text/html
Fonte: | Brazilian Journal of Medical and Biological Research  v.32 n.9 1999
Fonte: | info:eu-repo/semantics/openAccess