Data/hora: 05/06/2024 13:28:13

Provedor de dados: 	56	
País: 	Brazil	
Título: 	A gasometric method to determine erythrocyte catalase activity	
Autores: 	Siqueira,A.J.S.; Remião,J.O.; Azevedo,A.M.P.; Azambuja,C.R.J.	
Data: 	1999-09-01	
Ano: 	1999	
Palavras-chave: 	Erythrocyte; Catalase assay; Gasometry.	
Resumo: 	We describe a new gasometric method to determine erythrocyte catalase activity by the measurement of the volume of oxygen produced as a result of hydrogen peroxide decomposition in a system where enzyme and substrate are separated in a special reaction test tube connected to a manometer and the reagents are mixed with a motor-driven stirrer. The position of the reagents in the test tube permits the continuous measurement of oxygen evolution from the time of mixing, without the need to stop the reaction by the addition of acid after each incubation time. The enzyme activity is reported as KHb, i.e., mg hydrogen peroxide decomposed per second per gram of hemoglobin (s-1 g Hb-1). The value obtained for catalase activity in 28 samples of hemolyzed human blood was 94.4 ± 6.17 mg H2O2 s-1 g Hb-1. The results obtained were precise and consistent, indicating that this rapid, simple and inexpensive method could be useful for research and routine work.	
Tipo: 	Info:eu-repo/semantics/article	
Idioma: 	Inglês	
Identificador: 	http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X1999000900006	
Editor: 	Associação Brasileira de Divulgação Científica	
Relação: 	10.1590/S0100-879X1999000900006	
Formato: 	text/html	
Fonte: 	Brazilian Journal of Medical and Biological Research  v.32 n.9 1999	
Fonte: 	info:eu-repo/semantics/openAccess