Data/hora: 06/06/2024 05:27:14

Provedor de dados: | 56
País: | Brazil
Título: | Regenerated rat skeletal muscle after periodic contusions
Autores: | Minamoto,V.B.; Bunho,S.R.; Salvini,T.F.
Data: | 2001-11-01
Ano: | 2001
Palavras-chave: | Skeletal muscle; Periodic contusion; Long-term regeneration; Rat.
Resumo: | In the present study we evaluated the morphological aspect and changes in the area and incidence of muscle fiber types of long-term regenerated rat tibialis anterior (TA) muscle previously submitted to periodic contusions. Animals received eight consecutive traumas: one trauma per week, for eight weeks, and were evaluated one (N = 8) and four (N = 9) months after the last contusion. Serial cross-sections were evaluated by toluidine blue staining, acid phosphatase and myosin ATPase reactions. The weight of injured muscles was decreased compared to the contralateral intact one (one month: 0.77 ± 0.15 vs 0.91 ± 0.09 g, P = 0.03; four months: 0.79 ± 0.14 vs 1.02 ± 0.07 g, P = 0.0007, respectively) and showed abundant presence of split fibers and fibers with centralized nuclei, mainly in the deep portion. Damaged muscles presented a higher incidence of undifferentiated fibers when compared to the intact one (one month: 3.4 ± 2.1 vs 0.5 ± 0.3%, P = 0.006; four months: 2.3 ± 1.6 vs 0.3 ± 0.3%, P = 0.007, respectively). Injured TA evaluated one month later showed a decreased area of muscle fibers when compared to the intact one (P = 0.003). Thus, we conclude that: a) muscle fibers were damaged mainly in the deep portion, probably because they were compressed against the tibia; b) periodic contusions in the TA muscle did not change the percentage of type I and II muscle fibers; c) periodically injured TA muscles took four months to reach a muscle fiber area similar to that of the intact muscle.
Tipo: | Info:eu-repo/semantics/other
Idioma: | Inglês
Identificador: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2001001100012
Editor: | Associação Brasileira de Divulgação Científica
Relação: | 10.1590/S0100-879X2001001100012
Formato: | text/html
Fonte: | Brazilian Journal of Medical and Biological Research  v.34 n.11 2001
Fonte: | info:eu-repo/semantics/openAccess