Data/hora: 06/06/2024 13:13:49

Provedor de dados: | 56
País: | Brazil
Título: | Leishmania infantum heat shock protein 83 for the serodiagnosis of tegumentary leishmaniasis
Autores: | Celeste,B.J.; Angel,S.O.; Castro,L.G.M.; Gidlund,M.; Goto,H.
Data: | 2004-11-01
Ano: | 2004
Palavras-chave: | Leishmania infantum; Sp 83; ELISA; Serodiagnosis; Tegumentary leishmaniasis; Chagas' disease.
Resumo: | The serologic assay is an important tool in the diagnosis of leishmaniasis. One of the most commonly used tests is enzyme-linked immunosorbent assay (ELISA). Since total Leishmania promastigotes are used as antigen in the routine assay, false-positive reactions are frequent due to cross-reaction with sera from other diseases, mainly Chagas' disease. Therefore, an antigen that determines less cross-reactivity has been pursued for the serodiagnosis of leishmaniasis. In the present study we analyzed the use of recombinant Leishmania infantum heat shock protein (Hsp) 83 in ELISA for the serodiagnosis of cutaneous (N = 12) and mucocutaneous leishmaniasis (N = 14) and we observed the presence of anti-L. infantum Hsp 83 antibodies in all samples as well as anti-Leishmania total antigen antibodies. When cross-reactivity was tested, chronic Chagas' disease patients (N = 10) did not show any reactivity. Therefore, we consider this L. infantum Hsp 83 to be a good antigen for routine use for serodiagnosis of tegumentary leishmaniasis.
Tipo: | Info:eu-repo/semantics/other
Idioma: | Inglês
Identificador: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2004001100001
Editor: | Associação Brasileira de Divulgação Científica
Relação: | 10.1590/S0100-879X2004001100001
Formato: | text/html
Fonte: | Brazilian Journal of Medical and Biological Research  v.37 n.11 2004
Fonte: | info:eu-repo/semantics/openAccess