Data/hora: 16/06/2024 02:57:15

Provedor de dados: | 57
País: | Brazil
Título: | Histological processing techniques for the study of Dugesiidae development (Platyhelminthes, Tricladida, Paludicola)
Autores: | VARA,D. C. da; LEAL-ZANCHET,A. M.; LIZARDO-DAUDT,H. M.
Data: | 2001-05-01
Ano: | 2001
Palavras-chave: | Embryo development; Techniques; Glycol methacrilate; Tricladida.
Resumo: | The objective of the present study was to adapt techniques for the histological processing of Dugesiidae cocoons for the study of embryo development. The cocoons were fixed with formalin, SUSA, Bouin or paraformaldehyde/glutaraldehyde and subsequently embedded in Paraplast or glycol methacrylate (Historesin). Paraplast embedding yielded reasonable results only after the cocoon was perforated or fixed for a prolonged period of time using softening techniques with acid solutions. When the SUSA or Bouin fixative and Historesin embedding techniques were used the results were good for light microscopical analysis. Fixation with paraformaldehyde/glutaraldehyde and glycol methacrylate embedding resulted in better tissue preservation, and did not require prolonged fixation or softening techniques. Thus, we suggest this technique for light microscopical analysis of embryo development in Dugesiidae.
Tipo: | Info:eu-repo/semantics/article
Idioma: | Inglês
Identificador: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0034-71082001000200019
Editor: | Instituto Internacional de Ecologia
Relação: | 10.1590/S0034-71082001000200019
Formato: | text/html
Fonte: | Revista Brasileira de Biologia  v.61 n.2 2001
Fonte: | info:eu-repo/semantics/openAccess