Data/hora: 03/06/2024 10:00:27

Provedor de dados: | 58
País: | Brazil
Título: | Application of representational difference analysis to identify genomic differences between Bradyrhizobium elkanii and B. Japonicum species
Autores: | Soares,René Arderius; Passaglia,Luciane Maria Pereira.
Data: | 2010-12-01
Ano: | 2010
Palavras-chave: | Bradyrhizobium; Genome comparison; Nitrogen fixation; Representational difference analysis.
Resumo: | Bradyrhizobium elkanii is successfully used in the formulation of commercial inoculants and, together with B. japonicum, it fully supplies the plant nitrogen demands. Despite the similarity between B. japonicum and B. elkanii species, several works demonstrated genetic and physiological differences between them. In this work Representational Difference Analysis (RDA) was used for genomic comparison between B. elkanii SEMIA 587, a crop inoculant strain, and B. japonicum USDA 110, a reference strain. Two hundred sequences were obtained. From these, 46 sequences belonged exclusively to the genome of B. elkanii strain, and 154 showed similarity to sequences from B. japonicum genome. From the 46 sequences with no similarity to sequences from B. japonicum, 39 showed no similarity to sequences in public databases and seven showed similarity to sequences of genes coding for known proteins. These seven sequences were divided in three groups: similar to sequences from other Bradyrhizobium strains, similar to sequences from other nitrogen-fixing bacteria, and similar to sequences from non nitrogen-fixing bacteria. These new sequences could be used as DNA markers in order to investigate the rates of genetic material gain and loss in natural Bradyrhizobium strains.
Tipo: | Info:eu-repo/semantics/article
Idioma: | Inglês
Identificador: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822010000400036
Editor: | Sociedade Brasileira de Microbiologia
Relação: | 10.1590/S1517-83822010000400036
Formato: | text/html
Fonte: | Brazilian Journal of Microbiology  v.41 n.4 2010
Fonte: | info:eu-repo/semantics/openAccess