Data/hora: 13/06/2024 00:32:48

Provedor de dados: | 58
País: | Brazil
Título: | Amylase production by solid-state fermentation of agro-industrial wastes using Bacillus sp.
Autores: | Saxena,Rajshree; Singh,Rajni.
Data: | 2011-12-01
Ano: | 2011
Palavras-chave: | Solid State Fermentation; Agro-industrial wastes; Amylase; Enzyme optimization; Stability; Bacillus sp.
Resumo: | Solid state fermentation was carried out using various agro- industrial wastes with the best amylase producing strain isolated from soil. Different physicochemical conditions were varied for maximum enzyme production. The strain produced about 5400 units/g of amylase at 1:3 moisture content, 20% inoculum, after 72 h of incubation with Mustard Oil seed cake as the substrate. The optimum temperature and pH of the enzyme activity were found to be 50ºC and 6 respectively. The enzyme was found to be thermostable at 70ºC for about 2 h without any salt. It showed stability at pH range 5-7. The metal ions as Na+, Ca++, Mg++ and Co++ enhanced the enzyme activity.
Tipo: | Info:eu-repo/semantics/article
Idioma: | Inglês
Identificador: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822011000400014
Editor: | Sociedade Brasileira de Microbiologia
Relação: | 10.1590/S1517-83822011000400014
Formato: | text/html
Fonte: | Brazilian Journal of Microbiology  v.42 n.4 2011
Fonte: | info:eu-repo/semantics/openAccess