Data/hora: 01/06/2024 02:51:47

Provedor de dados: | 58
País: | Brazil
Título: | Carbon sources effect on pectinase production from Aspergillus japonicus 586
Autores: | Teixeira,Maria F. S.; Lima Filho,José L.; Durán,Nelson.
Data: | 2000-10-01
Ano: | 2000
Palavras-chave: | Pectinases; Aspergillus japonicus; Enzyme; Galacturonase; Pectinesterases.
Resumo: | The effect of different carbon sources on the pectinesterases, endo- and exo-polygalacturonase activities from Aspergillus japonicus 586 was evaluated in liquid media (Manachini solutions) supplemented with different substrate concentrations. The culture medium was inoculated with 5.10(6) spores/ml and mantained under agitation (140 rpm), at 30°C, during 122 h. The enzyme evaluation was carried out 24 h after filtration. The crude extract from A. japonicus 586 indicated that the best enzymatic activities were afforded in the presence of 0.5% pectin (pectinesterease), 0.2% pectin and 0.2% glycerol (endopolygalacturonase), and 0.5% pectin associated to 0.5% glucose (exopolygalacturonase). Carbon sources concentration, isolated or associated, significantly affects the pectinesterase, and endo- and exopolygalacturonase activities. Pectin, glucose and saccharose, when added to the culture medium in high concentrations, exhibited a repression effect on all the analyzed enzymes.
Tipo: | Info:eu-repo/semantics/article
Idioma: | Inglês
Identificador: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822000000400009
Editor: | Sociedade Brasileira de Microbiologia
Relação: | 10.1590/S1517-83822000000400009
Formato: | text/html
Fonte: | Brazilian Journal of Microbiology  v.31 n.4 2000
Fonte: | info:eu-repo/semantics/openAccess