Data/hora: 05/06/2024 14:02:55

Provedor de dados: | 58
País: | Brazil
Título: | A practical method for screening for beta-galactosidase secreting microbial colonies
Autores: | Barreto,Eriana S.; Silva,Daison O.; Passos,Flavia M. Lopes.
Data: | 2000-03-01
Ano: | 2000
Palavras-chave: | BIOTROL®; Beta-galactosidase.
Resumo: | Microbial colonies were replicated on YNB® agar plates overlaid with soft agar containing the glucose-oxidase/peroxidase (BIOTROL®) system. The pink color developed around the colonies was the result of the reaction of the glucose generated by the extracellular hydrolysis of lactose by beta-galactosidase, indicating secretion of this enzyme. This method proved to be very convenient for testing hundreds of colonies grown on agar plates for beta-galactosidase secretion by microbial cells.
Tipo: | Info:eu-repo/semantics/other
Idioma: | Inglês
Identificador: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822000000100009
Editor: | Sociedade Brasileira de Microbiologia
Relação: | 10.1590/S1517-83822000000100009
Formato: | text/html
Fonte: | Brazilian Journal of Microbiology  v.31 n.1 2000
Fonte: | info:eu-repo/semantics/openAccess