Data/hora: 02/06/2024 01:35:18

Provedor de dados: | 58
País: | Brazil
Título: | Expression of phenazine biosynthetic genes during the arbuscular mycorrhizal symbiosis of Glomus intraradices
Autores: | León-Martínez,Dionicia Gloria; Vielle-Calzada,Jean-Philippe; Olalde-Portugal,Víctor.
Data: | 2012-06-01
Ano: | 2012
Palavras-chave: | Mycorrhizal fungi; Glomus intradices; Phenazine; Biosynthesis.
Resumo: | To explore the molecular mechanisms that prevail during the establishment of the arbuscular mycorrhiza symbiosis involving the genus Glomus, we transcriptionally analysed spores of Glomus intraradices BE3 during early hyphal growth. Among 458 transcripts initially identified as being expressed at presymbiotic stages, 20% of sequences had homology to previously characterized eukaryotic genes, 30% were homologous to fungal coding sequences, and 9% showed homology to previously characterized bacterial genes. Among them, GintPbr1a encodes a homolog to Phenazine Biosynthesis Regulator (Pbr) of Burkholderia cenocepacia, an pleiotropic regulatory protein that activates phenazine production through transcriptional activation of the protein D isochorismatase biosynthetic enzyme phzD (Ramos et al., 2010). Whereas GintPbr1a is expressed during the presymbiotic phase, the G. intraradices BE3 homolog of phzD (BGintphzD) is transcriptionally active at the time of the establishment of the arbuscular mycorrhizal symbiosis. DNA from isolated bacterial cultures found in spores of G. intraradices BE3 confirmed that both BGintPbr1a and BGintphzD are present in the genome of its potential endosymbionts. Taken together, our results indicate that spores of G. intraradices BE3 express bacterial phenazine biosynthetic genes at the onset of the fungal-plant symbiotic interaction.
Tipo: | Info:eu-repo/semantics/article
Idioma: | Inglês
Identificador: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822012000200037
Editor: | Sociedade Brasileira de Microbiologia
Relação: | 10.1590/S1517-83822012000200037
Formato: | text/html
Fonte: | Brazilian Journal of Microbiology  v.43 n.2 2012
Fonte: | info:eu-repo/semantics/openAccess