Data/hora: 06/06/2024 17:17:00

Provedor de dados: | 58
País: | Brazil
Título: | Comparative study of two purified inulinases from thermophile Thielavia Terrestris NRRL 8126 and mesophile Aspergillus Foetidus NRRL 337 grown on Cichorium Intybus l
Autores: | Fawzi,Eman Mohamed.
Data: | 2011-06-01
Ano: | 2011
Palavras-chave: | Inulinases; Production; Purification; Thermophile; Thermostability.
Resumo: | Thirty fungal species grown on Cichorium intybus L. root extract as a sole carbon source, were screened for the production of exo-inulinase activities. The thermophile Thielavia terrestris NRRL 8126 and mesophile Aspergillus foetidus NRRL 337 gave the highest production levels of inulinases I &amp;amp; II at 50 and 24 ºC respectively. Yeast extract and peptone were the best nitrogen sources for highest production of inulinases I &amp;amp; II at five and seven days of incubation respectively. The two inulinases I &amp;amp; II were purified to homogeneity by gel-filtration and ion-exchange chromatography with 66.0 and 42.0 fold of purification respectively. The optimum temperatures of purified inulinases I &amp;amp; II were 75 and 50 ºC respectively. Inulinase I was more thermostable than the other one. The optimum pH for activity was found to be 4.5 and 5.5 for inulinases I &amp;amp; II respectively. A comparatively lower Michaelis-Menten constant (2.15 mg/ml) and higher maximum initial velocity (115 µmol/min/mg of protein) for inulinase I on inulin demonstrated the exoinulinase's greater affinity for inulin substrate. These findings are significant for its potential industrial application. The molecular mass of the inulinases I &amp;amp; II were estimated to be 72 &amp;amp; 78 kDa respectively by sodium dodecyl sulfate-polyacrylamide gel electrophoresis.
Tipo: | Info:eu-repo/semantics/article
Idioma: | Inglês
Identificador: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822011000200028
Editor: | Sociedade Brasileira de Microbiologia
Relação: | 10.1590/S1517-83822011000200028
Formato: | text/html
Fonte: | Brazilian Journal of Microbiology  v.42 n.2 2011
Fonte: | info:eu-repo/semantics/openAccess