Data/hora: 01/06/2024 14:24:25

Provedor de dados: | 58
País: | Brazil
Título: | Experimental infection with Brazilian Newcastle disease virus strain in pigeons and chickens
Autores: | Carrasco,Adriano de Oliveira Torres; Seki,Meire Christina; Benevenute,Jyan Lucas; Ikeda,Priscila; Pinto,Aramis Augusto.
Data: | 2016-03-01
Ano: | 2016
Palavras-chave: | Gallus gallus; Columba livia; Experimental infection; RT-PCR; Serology.
Resumo: | Abstract This study was designed with the goal of adding as much information as possible about the role of pigeons (Columba livia) and chickens (Gallus gallus) in Newcastle disease virus epidemiology. These species were submitted to direct experimental infection with Newcastle disease virus to evaluate interspecies transmission and virus-host relationships. The results obtained in four experimental models were analyzed by hemagglutination inhibition and reverse transcriptase polymerase chain reaction for detection of virus shedding. These techniques revealed that both avian species, when previously immunized with a low pathogenic Newcastle disease virus strain (LaSota), developed high antibody titers that significantly reduced virus shedding after infection with a highly pathogenic Newcastle disease virus strain (São Joao do Meriti) and that, in chickens, prevent clinical signs. Infected pigeons shed the pathogenic strain, which was not detected in sentinel chickens or control birds. When the presence of Newcastle disease virus was analyzed in tissue samples by RT-PCR, in both species, the virus was most frequently found in the spleen. The vaccination regimen can prevent clinical disease in chickens and reduce viral shedding by chickens or pigeons. Biosecurity measures associated with vaccination programs are crucial to maintain a virulent Newcastle disease virus-free status in industrial poultry in Brazil.
Tipo: | Info:eu-repo/semantics/article
Idioma: | Inglês
Identificador: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822016000100231
Editor: | Sociedade Brasileira de Microbiologia
Relação: | 10.1016/j.bjm.2015.07.001
Formato: | text/html
Fonte: | Brazilian Journal of Microbiology  v.47 n.1 2016
Fonte: | info:eu-repo/semantics/openAccess