Data/hora: 06/06/2024 06:11:21

Provedor de dados: 	58	
País: 	Brazil	
Título: 	The induction of differentially expressed proteins of Xylella fastidiosa with citrus extract	
Autores: 	Bellato,Cláudia de M.; Garcia,Andréia K.M.; Mestrinelli,Fabiana; Tsai,Siu M.; Machado,Marcos A.; Meinhardt,Lyndel W.	
Data: 	2004-09-01	
Ano: 	2004	
Palavras-chave: 	2DE; Callus; Pathogenicity; Protein; Xylella-citrus interaction.	
Resumo: 	An in vitro system was developed to induce and identify Xylella fastidiosa proteins that were differentially expressed in the presence of callus-derived extracts from its host, the citrus cultivar Pêra. To optimize the induction, we first developed a single culture medium for the growth of both, host and bacteria. This medium, CPXPm7, which mimics the citrus xylem sap, showed that X. fastidiosa at 72 h post-incubation had 10(8) colony forming units mL-1, while Pêra cells had the highest fresh weight content (0.79 g). After testing various methods of co-cultivation of the bacteria and host callus grown in this single medium, the best induction procedure was to grow X. fastidiosa in a solid medium amended with an extract of Pêra callus grown in CPXPm7. Analysis, by two-dimensional electrophoresis, of the X. fastidiosa proteins (120 µg of total proteins) grown in the presence of Pêra callus extract revealed 414 differentially expressed protein spots when compared to the protein profile obtained in the absence of the extract. The system developed in this study improves the induction and analysis of differentially expressed proteins of X. fastidiosa, which may be involved in pathogenicity.	
Tipo: 	Info:eu-repo/semantics/article	
Idioma: 	Inglês	
Identificador: 	http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822004000200012	
Editor: 	Sociedade Brasileira de Microbiologia	
Relação: 	10.1590/S1517-83822004000200012	
Formato: 	text/html	
Fonte: 	Brazilian Journal of Microbiology  v.35 n.3 2004	
Fonte: 	info:eu-repo/semantics/openAccess