Data/hora: 11/06/2024 07:20:20

Provedor de dados: | 58
País: | Brazil
Título: | Azole resistance in Candida spp. isolated from Catú Lake, Ceará, Brazil: an efflux-pump-mediated mechanism
Autores: | Brilhante,Raimunda S.N.; Paiva,Manoel A.N.; Sampaio,Célia M.S.; Castelo-Branco,Débora S.C.M.; Teixeira,Carlos E.C.; Alencar,Lucas P. de; Bandeira,Tereza J.P.G.; Monteiro,André J.; Cordeiro,Rossana A.; Pereira-Neto,Waldemiro A.; Sidrim,José J.C.; Moreira,José L.B.; Rocha,Marcos F.G.
Data: | 2016-03-01
Ano: | 2016
Palavras-chave: | Aquatic environments; Yeast microbiota; Candida spp.; Antifungal resistance.
Resumo: | Abstract Since, there is no study reporting the mechanism of azole resistance among yeasts isolated from aquatic environments; the present study aims to investigate the occurrence of antifungal resistance among yeasts isolated from an aquatic environment, and assess the efflux-pump activity of the azole-resistant strains to better understand the mechanism of resistance for this group of drugs. For this purpose, monthly water and sediment samples were collected from Catú Lake, Ceará, Brazil, from March 2011 to February 2012. The obtained yeasts were identified based on morphological and biochemical characteristics. Of the 46 isolates, 37 were Candida spp., 4 were Trichosporon asahii, 3 were Cryptococcus laurentii, 1 Rhodotorula mucilaginosa, and 1 was Kodamaea ohmeri. These isolates were subjected to broth microdilution assay with amphotericin B, itraconazole, and fluconazole, according to the methodology standardized by the Clinical and Laboratory Standards Institute (CLSI). The minimum inhibitory concentrations (MICs) of amphotericin B, itraconazole, and fluconazole were 0.03125–2 µg/mL, 0.0625 to ≥16 µg/mL, and 0.5 to ≥64 µg/mL, respectively, and 13 resistant azole-resistant Candida isolates were detected. A reduction in the azole MICs leading to the phenotypical reversal of the azole resistance was observed upon addition of efflux-pump inhibitors. These findings suggest that the azole resistance among environmental Candida spp. is most likely associated with the overexpression of efflux-pumps.
Tipo: | Info:eu-repo/semantics/article
Idioma: | Inglês
Identificador: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822016000100033
Editor: | Sociedade Brasileira de Microbiologia
Relação: | 10.1016/j.bjm.2015.11.008
Formato: | text/html
Fonte: | Brazilian Journal of Microbiology  v.47 n.1 2016
Fonte: | info:eu-repo/semantics/openAccess