Data/hora: 05/06/2024 19:04:58

Provedor de dados: | 58
País: | Brazil
Título: | Carbapenem-resistant Pseudomonas aeruginosa: association with virulence genes and biofilm formation
Autores: | Gonçalves,Iara Rossi; Dantas,Raquel Cristina Cavalcanti; Ferreira,Melina Lorraine; Batistão,Deivid William da Fonseca; Gontijo-Filho,Paulo Pinto; Ribas,Rosineide Marques.
Data: | 2017-06-01
Ano: | 2017
Palavras-chave: | Pseudomonas aeruginosa; Resistance; Metallo-β-lactamases; Virulence; Biofilm.
Resumo: | Abstract Pseudomonas aeruginosa is an opportunistic pathogen that causes frequently nosocomial infections, currently becoming more difficult to treat due to the various resistance mechanisms and different virulence factors. The purpose of this study was to determine the risk factors independently associated with the development of bacteremia by carbapenem-resistant P. aeruginosa, the frequency of virulence genes in metallo-β-lactamases producers and to evaluate their ability to produce biofilm. We conducted a case–control study in the Uberlândia Federal University – Hospital Clinic, Brazil. Polymerase Chain Reaction was performed for metallo-β-lactamases and virulence genes. Adhesion and biofilm assays were done by quantitative tests. Among the 157 strains analyzed, 73.9% were multidrug-resistant, 43.9% were resistant to carbapenems, 16.1% were phenotypically positive for metallo-β-lactamases, and of these, 10.7% were positive for blaSPM gene and 5.3% positive for blaVIM. The multivariable analysis showed that mechanical ventilation, enteral/nasogastric tubes, primary bacteremia with unknown focus, and inappropriate therapy were independent risk factors associated with bacteremia. All tested strains were characterized as strongly biofilm producers. A higher mortality was found among patients with bacteremia by carbapenem-resistant P. aeruginosa strains, associated independently with extrinsic risk factors, however it was not evident the association with the presence of virulence and metallo-β-lactamases genes.
Tipo: | Info:eu-repo/semantics/article
Idioma: | Inglês
Identificador: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822017000200211
Editor: | Sociedade Brasileira de Microbiologia
Relação: | 10.1016/j.bjm.2016.11.004
Formato: | text/html
Fonte: | Brazilian Journal of Microbiology  v.48 n.2 2017
Fonte: | info:eu-repo/semantics/openAccess