Data/hora: 10/06/2024 12:06:41

Provedor de dados: 	58	
País: 	Brazil	
Título: 	Binding effect of proline-rich-proteins (PRPs) on in vitro antimicrobial activity of the flavonoids	
Autores: 	Ansari,Jawaad Ahmed; Naz,Shahina; Tarar,Omer Mukhtar; Siddiqi,Rahmanullah; Haider,Muhammad Samee; Jamil,Khalid.	
Data: 	2015-03-01	
Ano: 	2015	
Palavras-chave: 	Proline rich proteins; Flavonoids; Catechin; Myrecetin; Kaempferol; Pelargonidin; Cyanidin; Gelatin; Casein; Binding constants.	
Resumo: 	The interaction of the cyanidin, pelargonidin, catechin, myrecetin and kaempferol with casein and gelatin, as proline rich proteins (PRPs), was studied. The binding constants calculated for both flavonoid-casein and flavonoid-gelatin were fairly large (105–107 M−1) indicating strong interaction. Due to higher proline content in gelatin, the binding constants of flavonoid-gelatin (2.5 × 105–6.2 × 107M−1) were found to be higher than flavonoid-casein (1.2 × 105–5.0 × 107 M−1). All the flavonoids showed significant antibacterial activity against the tested strains. Significant loss in activity was observed due to the complexation with PRPs confirming that binding effectively reduced the concentration of the free flavonoids to be available for antibacterial activity. The decline in activity was corresponding to the values of the binding constants. Though the activities of free catechin and myrecetin were higher compared to pelargonidin, cyanidin and kaempferol yet the decline in activity of catechin and myrecetin due to complexation with casein and gelatin was more pronounced.	
Tipo: 	Info:eu-repo/semantics/article	
Idioma: 	Inglês	
Identificador: 	http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822015000100183	
Editor: 	Sociedade Brasileira de Microbiologia	
Relação: 	10.1590/S1517-838246120130280	
Formato: 	text/html	
Fonte: 	Brazilian Journal of Microbiology  v.46 n.1 2015	
Fonte: 	info:eu-repo/semantics/openAccess