Data/hora: 11/06/2024 10:31:56

Provedor de dados: 	58	
País: 	Brazil	
Título: 	Semi-scale production of PHAs from waste frying oil by Pseudomonas fluorescens S48	
Autores: 	Gamal,Rawia F.; Abdelhady,Hemmat M.; Khodair,Taha A.; El-Tayeb,Tarek S.; Hassan,Enas A.; Aboutaleb,Khadiga A.	
Data: 	2013-01-01	
Ano: 	2013	
Palavras-chave: 	Pseudomonas fluorescens S48; Biopolymer; PHAs; Batch; Two-stage batch; High-celldensity fed batch; Bioreactor; Recovery.	
Resumo: 	The present study aimed at developing a strategy to improve the volumetric production of PHAs by Pseudomonas fluorescens S48 using waste frying oil (WFO) as the sole carbon source. For this purpose, several cultivations were set up to steadily improve nutrients supply to attain high cell density and high biopolymer productivity. The production of PHAs was examined in a 14 L bioreactor as one-stage batch, two-stage batch, and high-cell-density fed-batch cultures. The highest value of polymer content in one-stage bioreactor was obtained after 60 h (33.7%). Whereas, the two-stage batch culture increased the polymer content to 50.1% after 54 h. High-cell-density (0.64 g/L) at continuous feeding rate 0.55 mL/l/h of WFO recorded the highest polymer content after 54 h (55.34%). Semi-scale application (10 L working volume) increased the polymer content in one-stage batch, two-stage batch and high cell density fed-batch cultures by about 12.3%, 5.8% and 11.3%, respectively, as compared with that obtained in 2 L fermentation culture. Six different methods for biopolymer extraction were done to investigate their efficiency for optimum polymer recovery. The maximum efficiency of solvent recovery of PHA was attained by chloroform-hypochlorite dispersion extraction. Gas chromatography (GC) analysis of biopolymer produced by Pseudomonas fluorescens S48 indicated that it solely composed of 3-hydrobutyric acid (98.7%). A bioplastic film was prepared from the obtained PHB. The isolate studied shares the same identical sequence, which is nearly the complete 16S rRNA gene. The identity of this sequence to the closest pseudomonads strains is about 98-99%. It was probably closely related to support another meaningful parsiomony analysis and construction of a phylogenetic tree. The isolate is so close to Egyptian strain named EG 639838.	
Tipo: 	Info:eu-repo/semantics/article	
Idioma: 	Inglês	
Identificador: 	http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822013000200034	
Editor: 	Sociedade Brasileira de Microbiologia	
Relação: 	10.1590/S1517-83822013000200034	
Formato: 	text/html	
Fonte: 	Brazilian Journal of Microbiology  v.44 n.2 2013	
Fonte: 	info:eu-repo/semantics/openAccess