Data/hora: 11/06/2024 07:55:22

Provedor de dados: 	58	
País: 	Brazil	
Título: 	Characterization of biotechnologically relevant extracellular lipase produced by Aspergillus terreus NCFT 4269.10	
Autores: 	Sethi,Bijay Kumar; Nanda,Prativa Kumari; Sahoo,Santilata.	
Data: 	2016-03-01	
Ano: 	2016	
Palavras-chave: 	Aspergillus terreus; Lipase; Liquid static surface fermentation; Solid-state fermentation.	
Resumo: 	Abstract Enzyme production by Aspergillus terreus NCFT 4269.10 was studied under liquid static surface and solid-state fermentation using mustard oil cake as a substrate. The maximum lipase biosynthesis was observed after incubation at 30 °C for 96 h. Among the domestic oils tested, the maximum lipase biosynthesis was achieved using palm oil. The crude lipase was purified 2.56-fold to electrophoretic homogeneity, with a yield of 8.44%, and the protein had a molecular weight of 46.3 kDa as determined by SDS-PAGE. Enzyme characterization confirmed that the purified lipase was most active at pH 6.0, temperature of 50 °C, and substrate concentration of 1.5%. The enzyme was thermostable at 60 °C for 1 h, and the optimum enzyme–substrate reaction time was 30 min. Sodium dodecyl sulfate and commercial detergents did not significantly affect lipase activity during 30-min incubation at 30 °C. Among the metal ions tested, the maximum lipase activity was attained in the presence of Zn2+, followed by Mg2+ and Fe2+. Lipase activity was not significantly affected in the presence of ethylenediaminetetraacetic acid, sodium lauryl sulfate and Triton X-100. Phenylmethylsulfonyl fluoride (1 mM) and the reducing, β-mercaptoethanol significantly inhibited lipase activity. The remarkable stability in the presence of detergents, additives, inhibitors and metal ions makes this lipase unique and a potential candidate for significant biotechnological exploitation.	
Tipo: 	Info:eu-repo/semantics/article	
Idioma: 	Inglês	
Identificador: 	http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822016000100143	
Editor: 	Sociedade Brasileira de Microbiologia	
Relação: 	10.1016/j.bjm.2015.11.026	
Formato: 	text/html	
Fonte: 	Brazilian Journal of Microbiology  v.47 n.1 2016	
Fonte: 	info:eu-repo/semantics/openAccess