Data/hora: 06/06/2024 00:52:04

Provedor de dados: 	58	
País: 	Brazil	
Título: 	Optimization of exopolysaccharides production from a novel strain of Ganoderma lucidum CAU5501 in submerged culture	
Autores: 	Yuan,Baojing; Chi,Xiaoyan; Zhang,Rijun.	
Data: 	2012-06-01	
Ano: 	2012	
Palavras-chave: 	Optimization; Exopolysaccharides; Ganoderma lucidum; Orthogonal matrix method; Submerged culture.	
Resumo: 	This study aimed at optimizing the medium of a new Ganoderma lucidum strain CAU5501 to enhance the yield of exopolysaccharides (EPS) and mycelial growth. Firstly, the suitable level of glucose, magnesium, phosphate and C/N ratio was determined by single factor experiment. Subsequently, the optimum concentrations of these medium components were investigated using the orthogonal matrix method. The results indicated that the higher levels of EPS were correlated with the level of cell growth when glucose concentration was studied (data no show). The optimum medium for EPS yield was found to be 70 g/l glucose, 5 C/N ratio, 2.5 g/l KH2PO4, 0.75 g/l MgSO4·7H2O, and for mycelial growth was 50 g/l glucose, 5 C/N ratio, 1.5 g/l KH2PO4, 0.5 g/l MgSO4·7H2O. When cultivated in the obtained optimal media in 3 L shake flask, compared to the basal medium, the EPS yield increased markedly from 1.003 to 1.723 g/l, and the mycelium formation was also markedly improved from 2.028 to 7.235 g/l. Results obtained in this study are beneficial to further study for enhancing the production of Ganoderma lucidum polysaccharides in large scale commercialized production.	
Tipo: 	Info:eu-repo/semantics/article	
Idioma: 	Inglês	
Identificador: 	http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822012000200009	
Editor: 	Sociedade Brasileira de Microbiologia	
Relação: 	10.1590/S1517-83822012000200009	
Formato: 	text/html	
Fonte: 	Brazilian Journal of Microbiology  v.43 n.2 2012	
Fonte: 	info:eu-repo/semantics/openAccess