Data/hora: 18/06/2024 00:49:55

Provedor de dados: | 58
País: | Brazil
Título: | Optimization of flask culture medium and conditions for hyaluronic acid production by a streptococcus equisimilis mutant nc2168
Autores: | Chen,Yong-Hao; Li,Jun; Liu,Li; Liu,Hong-Zhi; Wang,Qiang.
Data: | 2012-12-01
Ano: | 2012
Palavras-chave: | Streptococcus equisimilis mutant; Hyaluronic acid; Optimization; Culture medium; Culture conditions.
Resumo: | A mutant designated NC2168, which was selected from wild-type Streptococcus equisimilis CVCC55116by ultraviolet ray combined with60Co-γ ray treatment and does not produce streptolysin, was employed to produce hyaluronic acid (HA). In order to increase the output of HA in a flask, the culture medium and conditions for NC2168 were optimized in this study. The influence of culture medium ingredients including carbon sources, nitrogen sources and metal ions on HA production was evaluated using factional factorial design. The mathematical model, which represented the effect of each medium component and their interaction on the yield of HA, was established by the quadratic rotary combination design and response surface method. The model estimated that, a maximal yield of HA could be obtained when the concentrations of yeast extract, peptone, glucose, and MgSO4 were set at 3 g/100 mL, 2 g/100 mL, 0.5 g/100 mL and 0.15 g/100 mL, respectively. Compared with the values obtained by other runs in the experimental design, the optimized medium resulted in a remarkable increase in the output of HA and the maximum of the predicted HA production was 174.76 mg/L. The model developed was accurate and reliable for predicting the production of HA by NC2168.Cultivation conditions were optimized by an orthogonal experimental design and the optimal conditions were as follows: temperature 33ºC, pH 7.8, agitation speed 200 rpm, medium volume 20 mL.
Tipo: | Info:eu-repo/semantics/article
Idioma: | Inglês
Identificador: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822012000400040
Editor: | Sociedade Brasileira de Microbiologia
Relação: | 10.1590/S1517-83822012000400040
Formato: | text/html
Fonte: | Brazilian Journal of Microbiology  v.43 n.4 2012
Fonte: | info:eu-repo/semantics/openAccess