Data/hora: 01/06/2024 07:33:29

Provedor de dados: 	58	
País: 	Brazil	
Título: 	Isolation and characterization of Caulobacter mutants impaired in adaptation to stationary phase	
Autores: 	Italiani,Valéria C. S.; Marques,Marilis V.	
Data: 	2003-04-01	
Ano: 	2003	
Palavras-chave: 	Stationary phase; Stress; Transposon; Bacteria.	
Resumo: 	The entry into stationary phase causes a change in the pattern of gene expression of bacteria, when the cells must express a whole set of genes involved mainly with resistance to starvation and to environmental stresses. As an attempt to identify genes important for the survival of Caulobacter crescentus in stationary phase, we have screened a library of 5,000 clones generated by random transposon mutagenesis for mutants that showed reduced viability after prolonged growth. Four clones were selected, which displayed either lower viability or a longer time of recovery from stationary phase. The genes disrupted were identified, and the gene products were found to be mainly involved with amino acid metabolism (glutamate N-acetyltransferase, 4-hydroxyphenylpyruvate dioxygenase and L-aspartate oxidase) or with recombination (exonuclease RecJ). Each mutant was tested for resistance to stresses, such as oxidative, saline, acidic, heat and UV exposure, showing different responses. Although the mutations obtained were not in genes involved specifically in stationary phase, our results suggest that amino acids metabolism may play an important role in keeping viability during this growth phase.	
Tipo: 	Info:eu-repo/semantics/article	
Idioma: 	Inglês	
Identificador: 	http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822003000100018	
Editor: 	Sociedade Brasileira de Microbiologia	
Relação: 	10.1590/S1517-83822003000100018	
Formato: 	text/html	
Fonte: 	Brazilian Journal of Microbiology  v.34 n.1 2003	
Fonte: 	info:eu-repo/semantics/openAccess