Data/hora: 06/06/2024 10:11:54

Provedor de dados: 	58	
País: 	Brazil	
Título: 	ERIC-PCR genotypic characterization of Haemophilus parasuis isolated from Brazilian swine	
Autores: 	Moreno,Luisa Zanolli; Castilla,Karina Salvagni; Gobbi,Débora Dirani Sena de; Coutinho,Tania Alen; Ferreira,Thais Sebastiana Porfida; Moreno,Andrea Micke.	
Data: 	2011-12-01	
Ano: 	2011	
Palavras-chave: 	Haemophilus parasuis; Glässer disease; Genotyping; ERIC-PCR.	
Resumo: 	Haemophilus parasuis infection, known as Glässer's disease, is characterized by fibrinous polyserositis, arthritis and meningitis in piglets. Although traditional diagnosis is based on herd history, clinical signs, bacterial isolation and serotyping, the molecular-based methods are alternatives for species-specific tests and epidemiologic study. The aim of this study was to characterize H. parasuis strains isolated from different states of Brazil by serotyping, PCR and ERIC-PCR. Serotyping revealed serovar 4 as the most prevalent (24 %), followed by serovars 14 (14 %), 5 (12 %), 13 (8 %) and 2 (2 %), whereas 40 % of the strains were considered as non-typeable. From 50 strains tested 43 (86%) were positive to Group 1 vtaA gene that have been related to virulent strains of H.parasuis. ERIC-PCR was able to type isolates tested among 23 different patterns, including non-typeable strains. ERIC-PCR patterns were very heterogeneous and presented high similarity between strains of the same animal or farm origin. The results indicated ERIC-PCR as a valuable tool for typing H. parasuis isolates collected in Brazil.	
Tipo: 	Info:eu-repo/semantics/article	
Idioma: 	Inglês	
Identificador: 	http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822011000400025	
Editor: 	Sociedade Brasileira de Microbiologia	
Relação: 	10.1590/S1517-83822011000400025	
Formato: 	text/html	
Fonte: 	Brazilian Journal of Microbiology  v.42 n.4 2011	
Fonte: 	info:eu-repo/semantics/openAccess